A nucleoside triphosphate for site-specific labelling of DNA by the Staudinger ligation
نویسندگان
چکیده
منابع مشابه
A nucleoside triphosphate for site-specific labelling of DNA by the Staudinger ligation.
A novel nucleotide building block for enzymatic synthesis of azide modified DNA and subsequent conjugation via Staudinger ligation was developed.
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The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protei...
متن کاملGeneral method for site-specific protein immobilization by Staudinger ligation.
Protein microarrays are playing an increasingly important role in the discovery and characterization of protein-ligand interactions. The uniform orientation conferred by site-specific immobilization is a demonstrable advantage in using such microarrays. Here, we report on a general strategy for fabricating gold surfaces displaying a protein in a uniform orientation. An azido group was installed...
متن کاملDNA conjugation by Staudinger ligation.
Two 5 modified 2'-deoxyuridin triphosphates and a 7 modified 2'-deoxy-7-deazaadenosine were synthesized carrying a terminal azide linked to the base. For probing the sterical influence on incorporation and Staudinger ligation different sized flexible linkers were chosen. All three nucleotides can completely replace their natural counterparts in primer extension as well as polymerase chain react...
متن کاملDNA Conjugation by the Staudinger Ligation: New Thymidine Analogues
Two novel modified 2'-deoxyuridine triphosphates carrying an azide functionality linked to the nucleobase were synthesized. For probing the sterical influence on enzymatic incorporation and Staudinger ligation, differently sized flexible linkers were chosen. Both nucleotides can completely replace natural thymidine in primer extension as well as polymerase chain reaction (PCR) using Pyrococcus ...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2007
ISSN: 1359-7345,1364-548X
DOI: 10.1039/b618257g